Introduction to Optical Microscopy

  • Mertz, Jerome (Boston University)
Copyright Year:2010
ISBN:9780981519487
Specifications:432 pages, hardback, printed in black and white

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Introduction to Optical Microscopy

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About this Title

Introduction to Optical Microscopy provides a rigorous and comprehensive overview of the fundamentals of optical microscopy. Starting from basic principles in Fourier optics, partial coherence, 3D imaging theory, and the physics of scattering and fluorescence, Introduction to Optical Microscopy explores a broad range of microscopy techniques. These include classical techniques such as phase contrast, confocal microscopy, etc., and progress to more modern techniques such as holographic microscopy, optical coherence tomography, two-photon microscopy, coherent anti-Stokes Raman scattering microscopy, etc.. The final chapters present a survey of new directions, including structured illumination and superresolution. Introduction to Optical Microscopy is designed to provide a solid theoretical foundation for graduate students or researchers who want to enter the field. While valuable as a reference, it can also serve as a textbook, as it includes a corresponding website that provides problem sets and an instructor’s solution manual.

About the Author

Jerome Mertz, PhD, is professor of Biomedical Engineering and director of the Biomicroscopy Laboratory at Boston University. Prior to joining Boston University, he was a CNRS researcher at the École Supérieure de Physique et de Chimie Industrielles in Paris. He specializes in the development of novel microscopy techniques for biological imaging.

Table of Contents

1 Introduction
2 Monochromatic wave propagation
3 Monocrhomatic field propagation through lens
4. Intensity propagation
5 3D imaging
6 Radiomety
7 Intensity fluctuations
8 Detection noise
9 Absorption and scattering
10 Phase contrast
11 Holographic microscopy
12 Optical coherance tomography
13 Fluorescence
14 Confocal microscopy
15 Two-photon microscopy
16 Coherent nonlinear microscopy
17 Structured illumination microscopy
18 Superresolution
A. Notations and conventions
Index

Supplements

The problem set and errata are available at the author's site. Please contact the publisher at info@roberts-publishers.com for the instructor's solutions.